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The amounts of p -nitroaniline released from -glutamyl- p -nitroanilide in assays using crude protein extracts from yeast expressing AsGGT1, AsGGT2, and AsGGT3, respectively, were significantly higher than that in assays using crude protein extracts from control yeast ( Table 1 ), indicating that the recombinant proteins of AsGGT1, AsGGT2, and AsGGT3 were successfully expressed and folded to form mature functional GGT proteins that can utilize -glutamyl- p -nitroanilide as a -glutamyl donor substrate in yeast cells

However, sustained activation of the UPR can trigger the apoptotic pathway, leading to apoptosis of cells

Hodny, Z., Reinis, M., Hubackova, S., Vasicova, P

Lephart ED, Draelos ZD

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2014;9(3), e28085