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Cultures were maintained in a humidified atmosphere of 5% CO 2 at 37C for 24 h and then incubated for the next 24-h period in DMEM-10% HS additionally containing recombinant HGF that was treated with peroxynitrite with or without trisulfide pre-treatment (see Figure 1d,f)

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All study procedure was conducted in accordance with the declaration of Helsinki

PLoS One 11, e0152551

T-cell responses, predominantly mono-functional, are often low And variable between ICS And ELISPOT assays