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Similarly, an increase in ROS level due to function failure of a mitochondrial situated ATP-dependent protease FtSH4 contributes to leaf senescence by regulating the signalling and accumulation of WRKY-reliant salicylic acid (SA) [65]

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Remijsen Q, Berghe TV, Wirawan E, Asselbergh B, Parthoens E, De Rycke R, et al

Int J Mol Sci 24(4):3207 Wang Y, Wu L, Fu J, Lv H, Guan X, Xu L, Chen P, Gao C, Hou P, Ji M (2013) Hyperthyroid monkeys: a nonhuman primate model of experimental Graves disease

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Subsequently, the sections were incubated with secondary antibody (goat anti-rabbit), rinsed with phosphate buffer saline (PBS), stained with DAB, and hematoxylin and eosin (H&E) staining, dehydrated, transparentized, and mounted before microscopic analysis