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QMP enhances cellular recycling and plasma half-life despite the absence of L585 To study the importance of L585 in a cellular context, we performed a human endothelial cell-based recycling assay (HERA) 48 using human microvascular endothelial cells (HMEC-1) that overexpress human FcRn (HMEC-1-FcRn) (Fig

Briefly, 3 g of each protein sample was mixed with 15 L of 50 mM ammonium bicarbonate (pH 7.8) (Thermo Fisher Scientific), followed by protein reduction with 2 L of 100 mM dithiothreitol (Thermo Fisher Scientific) for 30 min and protein alkylation with 5 L of 2-iodoacetamide (Thermo Fisher Scientific) for an additional 30 min at room temperature in the dark

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Perform sample pretreatment (5% SSA treatment) quickly as in 2.

Random scheduling creates peaks and valleys that may reduce effectiveness

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