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The rats were anesthetized with 3 to 4% isoflurane inhalation in an anesthesia chamber, and orally intubated to facilitate artificial ventilation

Quantification of absorbance of the blots was performed using densitometric scanning with a Fusion-FX7 system (Vilber Lourmat, Collgien, France) with mean optical density of the samples normalized to that of GAPDH

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Ultrasound Guided Injection Therapy If your pain is not responding to the measures above, then an ultrasound-guided injection might be helpful in the management

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SDSPAGE and western blotting Samples were fractioned by SDSPAGE on 8% gels under reducing conditions, immunoblotted onto nitrocellulose membranes (GE Healthcare) and bands detected by enhanced chemiluminescence (GE Healthcare) onto films developed on a Xograph Compact X5 processor