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Theyre mathematically linked by: G = RT ln(Kd) Example Suppose you have two drug candidates, A and B, targeting the same protein: - Drug A: Kd = 10^-9 M (1 nM), G = -12.8 kcal/mol - Drug B: Kd = 10^-6 M (1 M), G = -8.6 kcal/mol - Drug A has a lower Kd and more negative G, indicating higher binding affinity and stronger, more stable binding

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Upon germline removal, DAF-16/FOXO enters the intestinal nuclei and regulates downstream target genes 6

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The medication may be delivered into the lower abdomen, thighs, or arms, with the injection site rotating each week to avoid overuse