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The volume obtained was diluted in 1 ml of erythrocyte lysis buffer (8.3 g l 1 NH 4 Cl, 1 g l 1 KHCO 3 and 0.009% EDTA in H 2 O) and incubated for 5 min at 37 C

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The stability of IGF-1 LR3 under proper storage conditions allows researchers to plan long term experiments with confidence in compound efficacy throughout the study period