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Ms4a15 CRISPR homozygous mutations (30% efficiency) were generated in parental MF cells and validated by genotyping PCR and a 17-bp deletion in exon 2 by Tide (shinyapps.datacurators.nl/tide/)

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Kolkhof P, Jaisser F, Kim SY, Filippatos G, Nowack C, Pitt B (2017) Steroidal and novel non-steroidal mineralocorticoid receptor antagonists in heart failure and cardiorenal diseases: comparison at bench and bedside

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Figure 3 Potential Therapeutic Measures of TMAO for AS Reduced Production of TMAO to Protect the Heart TMAO is an intestinal microbiota-dependent metabolite, which is accumulated in the body by ingestion of large amounts of substances rich in phosphatidylcholine