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Reconstitution, Storage and Handling in Detail For laboratory use, the lyophilised powder is typically brought to room temperature before opening to avoid condensation, then reconstituted by adding sterile or bacteriostatic water slowly down the inside wall of the vial rather than directly onto the powder, and swirled gently rather than shaken to limit foaming and shear stress

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Protein bands were visualized using chemiluminescence (ECL, WBKLS0500, Millipore)

What happens if bacteriostatic water expires

Delrue C, Speeckaert MM