This combined approach is the most common protocol reported among experienced GHK-Cu users and makes intuitive sense given the complementary delivery profiles
It has a context-dependent answer
Measurement of ROS production Cells were washed in prewarmed PBS and treated with 10 M H 2 DCFDA for 30 min in dark
Source: The potential of GHK as an anti-aging peptide (2020) Claim 2: Intranasal administration bypasses the blood-brain barrier (BBB) to enhance central nervous system (CNS) delivery
Weidner KM, Behrens J, Vandekerckhove J, Birchmeier W
acknowledges support by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) within the cluster of excellence Precision medicine in chronic inflammation (DFG support code EXC2167), the collaborative research center Metaorganisms (DFG support code CRC1182), the research group miTarget (DFG support code FOR5042) and the German Ministry for Education and Research in the frame of iTREAT (BMBF support code 01ZX1902A)