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In animal models, this preservation was associated with increased fat oxidation relative to storage

After excluding 11 participants owing to excessive head motion, the analysis included 60 HC participants (34 women, 26 men

Kit Components Additional Materials Required Microplate reader capable of measuring absorbance at 340 nm at 25C Precision pipettes to deliver 2 L to 1 mL volumes Multi-channel pipettes to deliver 20 L to 200 L volumes Tubes to prepare sample dilutions 15 mL conical tubes Incubator at 25C Deionized water Assay Procedure Summary Prepare Working Solution

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This is why preservative-containing vials still carry post-puncture discard timelines in clinical practice