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Cells were harvested with trypsin digestion, pelleted, and washed with PBS before being analyzed by flow cytometry (BD Accuri C6)

For many people, all perceived threats can raise cortisol

Material preparation, data collection and analysis were performed by Ying Xiong, Te Liu, Juan Chen

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10.1007/s00408-023-00639-1 122 YangW.DingN.LuoR.ZhangQ.LiZ.ZhaoF.et al (2023)

Although the two domain mechanism is a generally accepted model for hormone binding and activation for class B GPCRs, different requirements of the ECD for the receptor activation exist among members of this family of GPCRs [27]