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Traditional use in Ayurvedic medicine Ayurvedic physicians documented shilajit's therapeutic applications over 2,000 years ago

10.1016/j.gene.2018.10.006 165 WangX.LiQ.XieT.YuanM.ShengX.QiX.et al (2021a)

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For protein precipitation and metabolite extraction, 25 l of plasma and diluted urine were added to 1.5 ml Eppendorf tubes, along with 225 l of methanol and 10 l of internal standard (IS) of both substances