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To ensure consumer safety and GRAS compliance, the final recombinant strains must be marker-free, without any foreign resistance genes or plasmid sequences (Koshi et al., 2022, Koshi et al., 2024)

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Before reaching the target dose, a typical dosing schedule may look like: Month 1: 0.25 mg once weekly for the first menth Month 2: 0.5 mg once weekly for the second month Month 3: 1 mg once weekly for the third month Month 4: 15 mg once weekly for the fourth month Target maintenance dose: 2.5 mg once weekly Some people may not be able to reach or tolerate the target dose due to side effects