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Cells were treated with different concentrations (from 1 to 1000 M) of a NO synthesis inhibitorL-NAMEduring different times (12, 24, 48, 72, 96, and 120 h), with and without retreatment every 24 h (retreatment was applied to approximate this in vitro model to the chronic exposure that occurs in vivo)

doi:10.1016/j.tcm.2021.12.008

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Divide the peptide amount in the vial (mg) by the volume of BAC water added (mL) to get the concentration in mg/mL