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Gastric banding: An adjustable silicone band filled with saline is wrapped around a part of the stomach, creating a small pouch that restricts the intake of food

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The cells were then centrifuged at 10,000 g for 2 min and incubated with 1 ml of 2 M HCl and 0.5% Triton X-100 (Sigma-Aldrich, 93443) for 30 min with gentle agitation