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Specifically, the effect allele encodes for leucine, which is more hydrophobic than the reference proline allele and may therefore increase stability of the hydrophobic region of the signal peptide (Supplementary Information)

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Lee WR, Shen SC, Liu CR, Fang CL, Hu CH, Fang JY

Beads were washed three times with 5 ml of wash buffer (50 mM NaH 2 PO 4 , 300 mM NaCl, 20 mM imidazole, 1 g ml 1 of leupeptin, 1 g ml 1 of AEBSF HCl and 1 l benzonase, pH 8.0)

Research shows that vitamin C is a required cofactor for two different enzymes in carnitine synthesis

Briefly, LX-2 cells were grown to a confluence of 60-70% on glass coverslips, washed three times with PBS, fixed in 4% formaldehyde for 20 min, permeabilized in 0.5% Triton X-100 for 5 min and blocked with Duolink blocking solution for 60 min at 37 C