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GHK binds copper because of a specific combination of functional groups that together form a high-affinity copper-coordination site: the N-terminal amine donates one nitrogen ligand, the histidine imidazole donates a second nitrogen ligand, and the deprotonated amide nitrogen of the Gly-His peptide bond donates a third nitrogen ligand

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(See Sharps Container below) If you do everything gently, your child will usually not awaken