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In summary, the high-performance liquid chromatography (HPLC) technique offers a higher level of sample analysis compared to high-performance thin-layer chromatography (HPTLC) due to its efficient separation time, as indicated in Table 4
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Feature selection was consistent across folds, with the same 12 metabolites repeatedly selected in more than 35 cross-validation folds, indicating stable model performance ( Figure 4F )
