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Incubate 30 min at RT

Finally, 20 L of a Latrunculin A solution was added and incubated for 10 minutes before starting the measurements 66

The protocol is straightforward: 0.25 to 0.5 mm needle depth for maintenance, 1.0 mm for anti-aging, 1.5 mm for scar revision, followed by immediate application of 0.05% GHK-Cu serum while micro-channels remain open (roughly 10 to 15 minutes)

DMSO-induced reduction in implantation rates and pregnancy losses were already observed from animal models 27

The flow-through sample was further loaded onto an anion-exchange membrane (Acrodisc unit with Mustang Q membrane