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The seeding medium consisted of DMEM (Gibco, #31966-021) supplemented with 10% v/v FBS (Gibco, #16140-071), 1% v/v Penicillin-Streptomycin (Gibco, #15140-122)

Indicate the\\ncharges at pH=7 for Look at the structure of oxidized glutathione below

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In vitiligo, elevated ROS levels and consequent LPO-derived products such as MDA and 4-HNE contribute to melanocyte cytotoxicity and function as DAMPs, triggering innate immune responses and promoting autoreactive T-cell recruitment, which further drives melanocyte destruction [85,86]

General handling: Protect from moisture, excessive heat, and light during routine laboratory handling

Then the tissue homogenate centrifuged at 2000 rpm/min for 15 min (Centrifuge 5804R, Eppendorf, Germany)