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RNA isolation and gene expression Jejunal RNA was isolated with TRIzol Reagent (Ambion) as per the manufacturers protocol and reverse transcribed using a High-Capacity cDNA Reverse Transcription Kit (Fisher Scientific 43-688-14)

PMCID: PMC4166708

When the medication is discontinued, appetite regulation returns toward baseline levels, and without the medication's effects, maintaining the same caloric restriction becomes more difficult

Kong G, Chew NW, Ng CH, Chin YH, Zeng R, Foo R, et al

Appl Cancer Res 37:14 Pant M, Lal A, Rana S, Rani A (2012) Plumbago zeylanica L.: a mini review