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doi:10.1093/ckj/sfae380
Int J Obes 44(9):18181831
Policymakers and industry have introduced initiatives to streamline the process
Injectable administration may cause temporary redness, swelling, or tenderness at the injection site
Preparation of protein lysates The cells collected from 6-well plates were lysed in 0.4 ml lysis buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, and 2% SDS) followed by sonication (10 cycles of 10 s pulses with 10 s rest intervals) using a Fisher Scientific Sonic Dismembrator Model 500 at 15% amplitude
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