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Additives : Antioxidants or reducing agents (e.g., DTT) may help, but use cautiously as they can interfere with some assays

When melanocytes are hyperactivated by specific triggers UV radiation, post-inflammatory signals from acne or injury, hormonal changes (especially estrogen and progesterone in melasma), or genetic predisposition they produce excess melanin that accumulates in patches, creating dark spots, uneven tone and visible discolouration

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doi: 10.2139/ssrn.5570323 57 YiSZhengBZhuYCaiYSunHZhouJ

[99], EpCAM was found as a novel Wnt axis target gene, which could be considered as a biomarker for this pathway activation