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1 mM phenylmethylsulfonyl fluoride, and 1% [w:v] polyvinyl-polyvinylpolypyrrolidone) and then centrifuged (12,000 g for 20 min at 4 C)

Bar graphs were generated using GraphPad Prism 7.04, 8 and 9.3.1 (GraphPad Software)

First, we performed extracellular flux analysis to assay the mitochondrial complex-dependent OCR profile in saponin-permeabilized Gclc fl/fl and Gclc fl/fl Mb1-Cre + FoB

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This combination: Neutralizes harmful free radicals that cause premature aging Protects DNA from oxidative stress Supports optimal cellular function and longevity Reduces inflammation markers throughout the body 2