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The restriction sites were introduced by PCR using the primer pairs 5-TGGATCCGCTCAAGAGTTTGTGAACTG-3 and 5-CTAAGCTTTTACTTGTACAGCTCGTCC-3 for GRX1-roGFP2iL and 5-AAGGATCCGTGAGCAAGGGCGAGGAGC-3 and 5-CTAAGCTTTTACTTGTACAGCTCGTCC-3 for roGFP2-iL

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Molecules 24 , 3593 (2019)