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Briefly, a polyclonal rat anti-mouse LY-6G antibody (1:2000, BD Pharmingen 551459), a polyclonal rabbit anti-rat antibody (1:100, Vector AI-4001) and an envision anti-rabbit HRP (Dako K4003) were used, followed by a diaminobenzidine (Dako K3468)) to reveal the peroxidase activity, and then counterstained with hematoxylin