The relative fold expression (2^ -ddCt value) was calculated using the Pfaffl method (Pfaffl et al., 2002)
After oxidase and 5% bovine serum albumin (BSA) closure, the sections were incubated with anti-TH primary antibody (1:500 dilution, Abcam, UK) at 4 C overnight
Fix: Replace unspecified codes with etiology-specific codes before submission
The top-scoring complex coordinate output file was selected based on the atomic interaction, atomic distance/clashing, binding interface with ligand (xx(lipoyl)lysine) and surface charges in the active site pocket following display of each coordinate in Coot
PubMed Edwards RG, Dewdney JM, Dobrzanski DL, Lee D
Use of any immunosuppressive or corticosteroid pharmacotherapy