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Peptides were precipitated with cold (4 C) diethyl ether and purified on an Agilent 1200 series High-Performance Liquid Chromatography (HPLC) instrument (1075% HPLC-grade acetonitrile for 20 min at a 2 mL/min flow rate over an Agilent Zorbax C18 column (5 m, 9.4 250 mm) tracked at 220, 254, and 280 nm

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(6) Note: All extraction reagents were pre-cooled at -20C before use

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