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Quantification of EdU-positive cells was performed using the Cell Proliferation Kit (C10337, Thermo Fisher Scientific) as previously described [20] and represented by the mean SEM of three independent experiments

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As a synthetic analog of human Glucagon-Like Peptide-1 (GLP-1), Semaglutide shares a 94% structural homology with native GLP-1 but includes key structural alterations that dramatically extend its pharmacokinetic profile

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Sublingual and IV Options Sublingual (under the tongue) and IV (intravenous) methods bypass the digestive system entirely