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The PCR products were digested with Nco I and Bam HI and ligated into the pKM260 expression vector (EUROSCARF, Frankfurt, Germany), which resulted in the N-terminal addition of a hexahistidine tag and linker with the sequence MHHHHHHASENLYFQGAM
Gastrointestinal Side Effects That May Affect Running Semaglutide commonly causes gastrointestinal (GI) side effects, including nausea, vomiting, bloating, constipation, and diarrhea
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J Nucl Med 36(12):22202228 Wang X, Xie C, Marathe CS et al (2020) Disparities in gastric emptying and postprandial glycaemia between Han Chinese and Caucasians with type 2 diabetes
Human recombinant GLP-1 and analogues have direct and indirect effects that are correlated with antiatherogenic properties, acting on signalling pathways in vascular smooth muscle cells [69]