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Griauzde, K.-P
-Catenin translocates to the nucleus, where it binds to a TCF/LEF transcription factor, repressing the expression of C/EBP and PPAR and, therefore, inhibiting adipogenesis [24]
Normal cells can adapt to these changes through established homeostatic mechanisms, while cancer cells, which rely on dysregulated metabolic pathways, experience significant growth inhibition and increased susceptibility to programmed cell death
Appetite suppression diminishes
Then, the blots were incubated with the following antibodies overnight at 4 C: mouse anti-GLP-1R (1:200, Santa Cruz, CA, USA), mouse anti-GLP-1 (1:100, Santa Cruz, CA, USA), mouse anti-CGRP (1:100, Santa Cruz, CA, USA), mouse anti-c-fos (1:200, Santa Cruz, CA, USA), rabbit anti-Iba-1 (1:1000, Abcam, Cambridge, MA, USA), rabbit anti-PI3K (1:1000, Proteintech, China), rabbit anti-Akt (1:1000, Proteintech, China), rabbit anti-phospho-Akt (1:2000, Cell Signalling, Boston, MA, USA), rabbit anti-IL-1 (1:800, Wanleibio, China), rabbit anti-TNF- (1:800, Wanleibio, China), and mouse anti--actin (1:1000, Beyotime, China)
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