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Cells were lysed in radioimmunoprecipitation assay buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate and 0.1% SDS) and protein content was measured using a standard BCA kit (Beyotime, P0009)

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The randomization list was concealed and remained inaccessible to study participants, investigators, monitors, sponsors, and laboratory staff until database lock

However, further heat needs to be supplied for the melting to take place: this is called the heat of fusion, and is an example of latent heat

However, H 2 concentrations in the air exceeding 4% pose an explosion risk