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Western blotting Cells were harvested and suspended in Total Lysis Buffer (0.125 M Tris-HCl pH 6.8, 4% SDS and 20% glycerol)

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For example, the poly(butadiene) m poly(l-lysine) n (m n = 107 200, 107 100, and 60 50), the micelle structures at physiological pH was swollen due to charge-charge repulsions to transit to helix-like nanostructures, releasing the cargo [263]