For immunofluorescence staining, murine lung slices were treated with antibodies against platelet endothelial cell adhesion molecule-1 (CD31) (1:500 dilution, Wuhan Servicebio Technology Co., LTD., China, GB15063-50), CD44 (1:500 dilution, Wuhan Servicebio Technology Co., LTD., China, GB112054), CD68 (1:2000, Wuhan Servicebio Technology Co., LTD., China, GB113109), -smooth muscle actin (-SMA) (1:500 dilution, Wuhan Servicebio Technology Co., LTD., China, GB111364), and amyloid precursor protein (APP) (1:500 dilution, Wuhan Servicebio Technology Co., LTD., China, GB11500)
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Recovery: Balance your workouts with rest to avoid overwhelming your antioxidant defenses
This issue arises from the difficulty in determining protein concentrations with sufficient accuracy to ensure precise 1:1 Fab:SpyCatcher coupling
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