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Chromatographic separation of these compounds was performed using L-colomn2 (Chemical Evaluation and Research Institute, Tokyo, Japan) with solvent A (0.1% formic acid in MQ water) and solvent B [0.1% formic acid in methanol (LC/MS grade, Fujifilm Wako Pure Chemical Corp.)] at a flow rate of 0.4 mL/min

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Recently, others have reported on this variation in practice, with some physicians estimating that 10-15% of GLP-1 patients are non-responders in their patient population

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