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Compounds were purified by flash column chromatography, using silica gel (Chromatogel 60 , 3575 m) as stationary phase

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The 1998 Raun characterisation paper documented Ipamorelin's selectivity profile GH release without prolactin, ACTH, cortisol, or aldosterone elevations characteristic of earlier GHRPs (PMID 9849822)

NS5B also has a low-affinity GTP-binding site, distal from the active site, which is thought to be an allosteric regulator of the fingerthumb interaction 47

Pull-down assays are a form of affinity purification very similar to immunoprecipitation, except that a "bait" protein is used instead of an antibody