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coli (Invitrogen, Cat#C601003) transformed with the Ulp1 expression plasmid were grown in Terrific Broth media supplemented with 100 g/ml carbenicillin at 37 C

Yes, when prescribed and monitored by a qualified provider at therapeutic doses

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[3] Eftekhari MH, Keshavarz SA, Jalali M, Elguero E, Eshraghian MR, Simondon KB

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