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In contrast to the synthesis, that occurs only intracellularly, the degradation or catabolic part of the GSH cycle, takes place partially extracellularly and partially inside cells

Due to the complexity of the kidney, as an organ containing specialized structures and multiple cell types, it becomes quite difficult to find a reliable model system to study the effects or toxicity of drugs and their metabolites on this organ

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However, while technical limitations may exist regarding adequate GSH rescue, we cannot rule out non-canonical and non-enzymatic functions of GCLC contributing to lipid-related phenotypes