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Blood was obtained via cardiac puncture and centrifuged at 4000 rpm at 4 C for 10 min to collect plasma

Other observations that support this effect include reports that lipedema is often first noticed at the time of puberty in young girls and occasionally following pregnancy, when multiple hormone levels are high, including estrogen

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J Thromb Haemost 3(2):405407

Plant Signal Behav 2:408409

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