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Western blot analysis Cell extracts were prepared with RIPA buffer containing proteases and phosphatase inhibitors and the concentration of cell lysates was determined using the Bradford protein assay reagent (Bio-Rad)

doi: 10.1016/j.jacbts.2018.09.004 25 DeanfieldJVermaSSciricaBMKahnSEEmersonSSRyanDet al

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(iii) the involvement of thiamine in acetylcholine (ACh) signaling and cholinergic activity in the enteric nervous system and non-neuronal cells of the gut and pancreas, employing both the coenzyme and non-coenzyme actions of thiamine