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Adding GHK-Cu during the remodeling phase is optional and more relevant for chronic conditions where tissue quality has deteriorated

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In obesity and insulin resistance, mitophagy markers, including LC3BII, Parkin, FUNDC1, and BNIP3, are generally reduced in both human and animal studies, reflecting compromised mitochondrial turnover

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In addition, investigators are exploring therapeutic strategies to address this metabolic feature of the disease

Cell proliferation and clonogenic assays The TACS XTT cell proliferation assay kit (Trevigen) was used for cell proliferation assays as described previously