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The calculated activation energies (G in kcal mol -1 ) for the abstraction the C2 proton of 7 to produce the cyanidin enolate ( 8-enolate) were found to be almost identical for the wild-type and the C13S variant, in agreement with their similar dehydratase activity measured experimentally

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- Completed a total of four human (external) application tests (including one trial using both ampoule and mist)

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however, caution is required in patients with shock and organ failure