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Cells were harvested, washed once with phosphate buffered saline (PBS, Gibco, #14190-094) and loosened from the cell flasks with Versene (Gibco, #15040-033) or TrypLE TM (Gibco, #12605-010)

In contrast, germline deletion of INHBA in mice is neonatally lethal, demonstrating the necessity of activin signaling during development 53

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