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The balance between two subsets of macrophages is known: the M1-like macrophages, which are associated with antimicrobial and pro-inflammatory activity, and the M2-like macrophages, which are mostly involved in tissue repair and are associated with an immune-modulatory activity

2.6 Chromatographic analysis of TMA-related compounds TMA and TMAO were measured through LC-MS/MS analysis using a system composed by an UHPLC Ultimate 3000 (Thermo Fisher Scientific) and a Q-Exactive Plus high-resolution mass spectrometer (Thermo Fisher Scientific)

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Look for reduced inflammation within the first week, visible wound size reduction by week 2-3, and accelerated re-epithelialization compared to similar past wounds

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