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Additional characterization may include peptide mapping, copper-content analysis, water determination, counterion assessment and component-specific recovery studies

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Glycolysis and the tricarboxylic acid (TCA) cycle utilize oxidized NAD as an electron acceptor to break down carbohydrates and generate NADH, while the mitochondrial electron transport chain (ETC) oxidizes NADH back to NAD, coupling this process to ATP synthesis 2