They're designed for precision, and their efficacy is intrinsically linked to how they're handled post-synthesis
Mittler and Zilinskas 1991)
However, if the tear was a radial tear, then Dr
Proper storage is important to preserve the effectiveness of the peptide for future use.
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10 mM DTT, protease-inhibitor (Thermo Fisher Scientific, Waltham, Massachusetts, United States) and PhosSTOP (Sigma-Aldrich) added to the extraction buffer just before use in a 50 mL polypropylene conical tube (Eppendorf, Hamburg, Germany), vortexed for 30 s and incubated at 65C for 30 min