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Excess secondary antibody was washed off by 3 consecutive 10-min rinses with PBST

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While the Clade 2 GS enzyme tested clearly has a slower rate of canonical enzyme activity than the Clade 1 GS, it can nevertheless synthesise glutathione: It has a high affinity for the -EC dipeptide substrate, and a preference for glycine as the terminal amino acid despite a lack of conservation in the two residues that apparently contribute to this specificity

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For a peptide reconstitution that needs to last, bacteriostatic water is the only rational choice