After 4 weeks of D-galactose injections, the D-Gal group mice received intraperitoneal injections of PBS every 2 days for 4 weeks, followed by tissue collection
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Similarly to total BDNF protein levels, the most common method for quantifying protein levels of pro- and mBDNF is enzyme-linked immunosorbent assay (ELISA), supporting their measurement based on previous findings 30,31,32
Scientific interest in GHK-Cu has expanded considerably as researchers continue exploring its interactions with gene expression, protein synthesis and molecular signalling