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Cells were washed three times with TBST and incubated in a mixture of two primary antibodies: 1 g ml 1 anti-human Met rat mAb and phospho-Met (Tyr1234/1235) (D26) XP rabbit mAb diluted by 1:1,000 in Can Get Signal Solution 1 for 1 h at 25 C

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Abstract Glucose homeostasis, regulated by glucose-dependent insulinotropic polypeptide (GIP), glucagon-like peptide-1 (GLP-1) and glucagon (GCG) is critical to human health